SOX10 Antibody (20B7) [NL557]

R&D Systems | Catalog # NL2864R

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Flow Cytometry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 20B7
View all SOX10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat SOX10
aa 1-118

Specificity

Detects human and rat SOX10. Epitope-mapping experiments indicated that clone 20B7 recognizes a determinant in the first 65 amino acids, which contains sequences unique to SOX10.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX10 Antibody (20B7) [NL557]

Detection of SOX10 in Ntera2.

SOX10 was detected in immersion fixed Ntera2 cells using Mouse Anti-Human/Rat SOX10 NorthernLights™ NL557-conjugated Monoclonal Antibody (Catalog # NL2864R) at a final concentration of 1X (1:10 dilution) for 3 hours at room temperature (A). Cells were counterstained with DAPI (blue, B). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for SOX10 Antibody (20B7) [NL557]

Application
Recommended Usage

Immunocytochemistry

1:10 dilution
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a 10X solution of antibody in 0.5 mL PBS containing 0.1% sodium azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: SOX10

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

References

  1. Lo, L. et al. (2002) Development 129:1553.

Long Name

SRY-box transcription factor 10

Alternate Names

DOM, PCWH, SOX-10, WS2E, WS4, WS4C

Entrez Gene IDs

6663 (Human); 20665 (Mouse); 29361 (Rat)

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (20B7) [NL557]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for SOX10 Antibody (20B7) [NL557]

For research use only

Citations for SOX10 Antibody (20B7) [NL557]

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