Human SART1 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB8034X Datasheet / COA / SDS

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FAB8034X-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SART1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SART1 in ELISAs and Western Blots. Detects mouse SART1 in Western Blots.

Source

Monoclonal Mouse IgG1 Clone # 865709

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human SART1
Asn647-Lys800
Accession # O43290

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SART1

Hypoxia-associated factor (HAF), also known as SART1, is a widely expressed 125 kDa intracellular protein that promotes assembly of the U2-type pre-mRNA spliceosome. SART1 is also an E3 ubiquitin ligase that promotes the ubiquitination and degradation of HIF-1 alpha. In contrast, it enhances the transcriptional activity of HIF-2 alpha. SART1 is upregulated in many tumor types and enables tumor growth during chronic hypoxia. Within amino acids 647-800, human SART1 shares 100% aa sequence identity with mouse and rat SART1.

Long Name

Squamous cell carcinoma antigen recognized by T cells

Entrez Gene IDs

9092 (Human); 20227 (Mouse); 29678 (Rat)

Alternate Names

Allergen Hom s 1; Ara1; HAF; HOMS1; hSART-1; hSnu66; hypoxia-associated factor; IgE Autoantigen; MGC2038; SART1; SART-1; SART1(259) protein; SART1(800) protein; SART1259; small nuclear ribonucleoprotein 110kDa (U4/U6.U5); SNRNP110; SNU66 homolog; Snu66; squamous cell carcinoma antigen recognised by T cells; Squamous cell carcinoma antigen recognized by T cells 1; squamous cell carcinoma antigen recognized by T cells; U4/U6.U5 tri-snRNP-associated 110 kDa protein; U4/U6.U5 tri-snRNP-associated protein 1