Human SMPD3 Antibody (758612)

R&D Systems | Catalog # MAB7184

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 758612
View all SMPD3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human SMPD3
Val2-Ala655
Accession # Q9NY59

Specificity

Detects human SMPD3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SMPD1 or rhPP2A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SMPD3 Antibody (758612)

Detection of Human SMPD3 antibody by Western Blot.

Detection of Human SMPD3 by Western Blot.

Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMPD3 Monoclonal Antibody (Catalog # MAB7184) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SMPD3 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SMPD3 Antibody (758612)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CEM human T-lymphoblastoid cell line

Reviewed Applications

Read 1 review rated 4 using MAB7184 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SMPD3

SMPD3 (Sphingomyelin phosphodiesterase 3; also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91% aa sequence identity with mouse SMPD3.

Long Name

Sphingomyelin Phosphodiesterase 3

Alternate Names

NSMASE2

Entrez Gene IDs

55512 (Human); 58994 (Mouse); 94338 (Rat)

Gene Symbol

SMPD3

UniProt

Q9NY59 (Human)

Additional SMPD3 Products

Product Documents for Human SMPD3 Antibody (758612)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SMPD3 Antibody (758612)

For research use only

Citations for Human SMPD3 Antibody (758612)

Customer Reviews for Human SMPD3 Antibody (758612) (1)

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  • Human SMPD3 Antibody
    Name: Sebastian Silvera
    Application: Western Blot
    Sample Tested: Skeletal muscle tissue
    Species: Mouse
    Verified Customer | Posted 08/22/2017
    2.5, 5, 10, and 20ug of protein loaded left to right, with ladder on far right
    SMPD3 in white Gastrocnemius muscle of mouse at protein concentrations of 2.5, 5, 10 and 20ug (Left to right). On PVDF membrane.
    Human SMPD3 Antibody (758612) MAB7184

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