Human SOX3 Antibody

R&D Systems | Catalog # MAB2569

R&D Systems
Discontinued Product
MAB2569 has been discontinued. View all SOX3 products.

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 287403
View all SOX3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOX3
Met1-Leu230
Accession # P41225

Specificity

Detects human SOX3 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human SOX2, 7, 10, or 17 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human SOX3 Antibody

SOX3 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

SOX3 in NTera‑2 Human Cell Line.

SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Mouse Anti-Human SOX3 Monoclonal Antibody (Catalog # MAB2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green, upper panel; Catalog # NL009) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human SOX3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB2569 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX3

SOX3 belongs to the Sox (SRY-like HMG-box gene) family of transcription factors with diverse roles in development. SOX3 is expressed in the central nervous system from the earliest stages of development. It is one of the earliest neural markers in vertebrates and plays a role in specifying neuronal fate.

Long Name

Transcription Factor SOX3

Alternate Names

SOXB

Entrez Gene IDs

6658 (Human)

Gene Symbol

SOX3

UniProt

P41225

Additional SOX3 Products

Product Documents for Human SOX3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SOX3 Antibody

For research use only

Customer Reviews for Human SOX3 Antibody (1)

5 out of 5
1 Customer Rating
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  • Human SOX3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: U87 glioma cells
    Species: Human
    Verified Customer | Posted 01/04/2022
    Human SOX3 Antibody MAB2569

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