Human SPRY3 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5735N Datasheet / COA / SDS

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AF5735N-100UG

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Human SPRY3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human SPRY3 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SPRY3
Met1-Glu142
Accession # O43610

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SPRY3

SPRY3 (sprouty homolog 3) is a member of the sprouty family of proteins. SPRY3 appears to modulate Ras/MAPK pathway signaling following RTK activation. Human SPRY3 is 288 amino acids (aa) in length and contains one SH2-binding domain (aa 25‑30), a PEST sequence (aa 82‑93), and a Cys-rich domain (aa 147‑270). SPRY3 forms homo- and hetero-oligomers with other SPRY family molecules. It also undergoes phosphorylation, ubiquitination and palmitoylation, the latter of which induces SPRY3 to associate with cell membranes.

Long Name

Sprouty Homolog 3

Entrez Gene IDs

10251 (Human); 236576 (Mouse)

Alternate Names

Antagonist Of FGF Signaling; HSPRY3; Protein Sprouty Homolog 3; sprouty (Drosophila) homolog 2; Sprouty Homolog 3 (Drosophila); SPRY3; spry-3