Human SPRY3 Antibody

Catalog #: AF5735 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5735
AF5735-SP

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Detection of Human SPRY3 by Western Blot.

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Human SPRY3 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human SPRY3 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SPRY3
Met1-Glu142
Accession # O43610

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SPRY3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human SPRY3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5735) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRY3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPRY3

SPRY3 (sprouty homolog 3) is a member of the sprouty family of proteins. SPRY3 appears to modulate Ras/MAPK pathway signaling following RTK activation. Human SPRY3 is 288 amino acids (aa) in length and contains one SH2-binding domain (aa 25‑30), a PEST sequence (aa 82‑93), and a Cys-rich domain (aa 147‑270). SPRY3 forms homo- and hetero-oligomers with other SPRY family molecules. It also undergoes phosphorylation, ubiquitination and palmitoylation, the latter of which induces SPRY3 to associate with cell membranes.

Long Name

Sprouty Homolog 3

Entrez Gene IDs

10251 (Human); 236576 (Mouse)

Alternate Names

Antagonist Of FGF Signaling; HSPRY3; Protein Sprouty Homolog 3; sprouty (Drosophila) homolog 2; Sprouty Homolog 3 (Drosophila); SPRY3; spry-3