Human TACE/ADAM17 Ectodomain Antibody (111608)

Detection of TACE/ADAM17 Ectodomain by Flow Cytometry

PLWH exhibited upregulated ADAM17 expression mediating CD16 downregulation.(A, B, C) PBMC from HIV-1-uninfected people (n = 5), and PLWH with (n = 6) or without ART (n = 5) were thawed and stained, and the percentages of ADAM17+ NK cells were calculated. (A) Representative flow plots from NK cells in healthy donors and PLWH are shown. (B) Summary boxplots for %ADAM17+ NK cells for total NK cells among groups were shown. (C) %ADAM17+ NK cells for total, CD56-CD16+ and CD56dim NK cells from PLWH (n = 11) were summarized in boxplot. (D, E) PBMC from PLWH with or without ART were cultured in the presence or absence of human IgG1 isotype, ADAM17-blocking antibody (aADAM17), or TAPI-1 (n = 11, respectively). Following overnight incubation, the percentages of CD16+ cells from live CD3-CD14-CD20- cells were calculated by flow cytometry. (D) Representative flow plots for live CD3-CD14-CD20- cells were depicted. (E) Shown were the summary boxplots for %CD16+ cells from CD3-CD14-CD20- cells where individual dots depict each subject (n = 5). (*; p<0.05, **; p<0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37669308), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human TACE / ADAM17 ELISA Standard Curve

Recombinant Human TACE/ADAM17 (Catalog # 930-ADB) was serially diluted and captured by Mouse Anti-Human TACE/ADAM17 Ectodomain Monoclonal Antibody (Catalog # MAB9304) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TACE/ADAM17 Ectodomain Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)