Human Thrombospondin-4 Antibody (276523)

R&D Systems | Catalog # MAB2390

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 276523
View all Thrombospondin-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-4
Ala22-Asn961 (Pro276Ala, Ala420Val)
Accession # P35443

Specificity

Detects human Thrombospondin-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human Thrombospondin-1, -2, or -3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Thrombospondin-4 Antibody (276523)

Detection of Human Thrombospondin-4 antibody by Western Blot.

Detection of Human Thrombospondin‑4 by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thrombospondin-4 Monoclonal Antibody (Catalog # MAB2390) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Thrombospondin-4 by Immunohistochemistry

Detection of Thrombospondin-4 by Immunohistochemistry

The picture of CAFs and NFs stained with Podoplanin and the western blot analysis of THBS4.(A), Representative picture of Podoplanin staining. Podoplanin was mainly stained at the cytoplasm fibroblasts. The expression level of Podoplanin was higher in CAFs, in compared with that in NFs. (B), THBS4 expression. CAFs expressed THBS4, but NFs and cancer cells did not. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31703077), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Thrombospondin-4 Antibody (276523)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human heart tissue

Reviewed Applications

Read 5 reviews rated 4 using MAB2390 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-4

Thrombospondin-4 (TSP-4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. TSP‑1 and -2 constitute subgroup A and form disulfide-linked homotrimers, whereas TSP-3, -4, and -5/COMP constitute subgroup B and form pentamers (1, 2). The human TSP-4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven TSP type-3 repeats (one with an RGD motif), and a TSP C-terminal domain (3). Human TSP-4 shares 93% aa sequence identity with mouse and rat TSP-4. Within the TSP type-3 repeats and the TSP C-terminal domain, human TSP-4 shares 79% aa sequence identity with TSP-3 and COMP, and 58% aa sequence identity with TSP-1 and -2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). TSP-4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of TSP-4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). TSP-4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). TSP-4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of TSP-4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type TSP-4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type TSP-4, although the A387P variant induces a greater release of proinflammatory molecules (13).

References

  1. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  2. Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
  3. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
  4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
  5. Hauser, N. et al. (1995) FEBS Lett. 368:307.
  6. Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
  7. Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
  8. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
  9. Stenina, O.I. et al. (2003) Circulation 108:1514.
  10. Topol, E.J. et al. (2001) Circulation 104:2641.
  11. Wessel, J. et al. (2004) Am. Heart J. 147:905.
  12. Stenina, O.I. et al. (2005) FASEB J. 19:1893.
  13. Pluskota, E. et al. (2005) Blood 106:3970.

Alternate Names

THBS4, Thrombospondin4, TSP-4

Entrez Gene IDs

7060 (Human); 21828 (Mouse)

Gene Symbol

THBS4

UniProt

P35443

Additional Thrombospondin-4 Products

Product Documents for Human Thrombospondin-4 Antibody (276523)

Certificate of Analysis

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Product Specific Notices for Human Thrombospondin-4 Antibody (276523)

For research use only

Citations for Human Thrombospondin-4 Antibody (276523)

Customer Reviews for Human Thrombospondin-4 Antibody (276523) (5)

4 out of 5
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Showing  1 - 5 的 5 reviews Showing All
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  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/01/2024
    Human Thrombospondin-4 Antibody (276523) MAB2390
  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    Human Thrombospondin-4 Antibody (276523) MAB2390
  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/04/2019
    We used this antibody for a sandwich ELISA in combination with pAb (AF2390)) and protein (2390-TH). This combination works very well for detecting the TSP4 in human serum and plasma
    Human Thrombospondin-4 Antibody (276523) MAB2390
  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/21/2019
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 23942617
    Species: Human
    Verified Customer | Posted 02/19/2015

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Associated Pathways

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