Human TLR10 Antibody (670719)

Detection of Human TLR10 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 2 µg/mL of Human TLR10 Monoclonal Antibody (Catalog # MAB6619) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TLR10 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human TLR10 by Western Blot

NETs promoted STING upregulation by activating TLR2 signaling. A KEGG analysis identified significantly altered pathways in control and NET-treated HUVECs. B Relative mRNA levels of TLRs in control and NET-treated HUVECs (n = 3). C Western blot images of TLRs expression in control and NET-treated HUVECs. D Representative images of immunofluorescence staining of TLR2 in control and NET-treated HUVECs. Scale bar: 30 μm. The expression of TLR2 was assessed by fluorescence intensity (n = 3). E The cell viability of HUVECs pretreated with C-29 (n = 3). F Relative mRNA levels of TLR2, STING, and TF in HUVECs pretreated with C-29 (n = 3). G Western blot images of STING pathway and TF expression in HUVECs pretreated with C-29. H Relative mRNA levels of STING in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi (n = 3). I Western blot images of TLR2 expression in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi. J Western blot was performed to analyze the levels of STING and TF expression in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi under stimulation of NETs. K Western blot images of TLR2 expression in HUVECs pretreated with DNase I. L Western blot images of TLR2 expression in murine lung tissues treated with DNase I. Each bar represents the mean ± SD. The comparison between the two groups was performed using unpaired t-tests (B, D, and H). Statistical analysis for three or more groups was carried out using 1-way ANOVA (E and F). *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37626060), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TLR10 by Western Blot

NETs promoted STING upregulation by activating TLR2 signaling. A KEGG analysis identified significantly altered pathways in control and NET-treated HUVECs. B Relative mRNA levels of TLRs in control and NET-treated HUVECs (n = 3). C Western blot images of TLRs expression in control and NET-treated HUVECs. D Representative images of immunofluorescence staining of TLR2 in control and NET-treated HUVECs. Scale bar: 30 μm. The expression of TLR2 was assessed by fluorescence intensity (n = 3). E The cell viability of HUVECs pretreated with C-29 (n = 3). F Relative mRNA levels of TLR2, STING, and TF in HUVECs pretreated with C-29 (n = 3). G Western blot images of STING pathway and TF expression in HUVECs pretreated with C-29. H Relative mRNA levels of STING in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi (n = 3). I Western blot images of TLR2 expression in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi. J Western blot was performed to analyze the levels of STING and TF expression in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi under stimulation of NETs. K Western blot images of TLR2 expression in HUVECs pretreated with DNase I. L Western blot images of TLR2 expression in murine lung tissues treated with DNase I. Each bar represents the mean ± SD. The comparison between the two groups was performed using unpaired t-tests (B, D, and H). Statistical analysis for three or more groups was carried out using 1-way ANOVA (E and F). *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37626060), licensed under a CC-BY license. Not internally tested by R&D Systems.