Human TNF RI/TNFRSF1A Antibody (925224)

Detection of Human TNF RI/TNFRSF1A by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB6251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of TNF RI/TNFRSF1A by Western Blot

NPS-1034 activates GADD45A, inhibits the MET and TNFRSF1A downstream signaling pathway proteins’ expression level, and upregulates the pro-apoptosis protein expression level in RCC cells. (A) Validation of NPS-1034, an MET/AXL inhibitor, on the MET downstream pathway protein expression level in RCC cells. (B) Assessment of the regulatory role of GADD45A and TNFR1-related pathways, such as the I kappa B alpha /NF kappa B signaling pathway. (C) The expression levels of several pro-apoptosis proteins were detected in RCC cells treated with NPS-1034 (0, 20, and 80 μM) for 48 h via Western blotting. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39451232), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of TNF RI/TNFRSF1A by Western Blot

The high expression of GADD45A correlates with a favorable prognosis and effect on the survival rate of patients with RCC. TNFRSF1A overexpression reversed the anticancer effects of NPS-1034 in RCC cells. (A) A volcano plot showing the differential expression of genes altered by NPS-1034 treatment in RCC cells. (B) Relative GADD45A mRNA expression in 877 patients. Kaplan–Meier plots showing the survival probabilities of RCC patients stratified by GADD45A expression. Patient survival details were derived from TCGA. (C) The increases in GADD45A-related pathway expression and the decreases in I kappa B alpha /NF kappa B-related pathway expression are all significantly reversed via TNFRSF1A overexpression. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39451232), licensed under a CC-BY license. Not internally tested by R&D Systems.