Human Transglutaminase 7/TGM7 Antibody (602708)

R&D Systems | Catalog # MAB5426

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 602708
View all Transglutaminase 7/TGM7 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 7/TGM7
Asp2-Pro710
Accession # Q96PF1

Specificity

Detects human Transglutaminase 7/TGM7 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human TGM2, 3, 4, or recombinant mouse TGM2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Transglutaminase 7/TGM7 Antibody (602708)

Detection of Human Transglutaminase 7/TGM7 antibody by Western Blot.

Detection of Human Transglutaminase 7/TGM7 by Western Blot.

Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Human Transglutaminase 7/TGM7 Monoclonal Antibody (Catalog # MAB5426) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Transglutaminase 7/TGM7 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Transglutaminase 7/TGM7 Antibody (602708)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: PC‑3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Transglutaminase 7/TGM7

Transglutaminase 7 (TGM7), also known as protein-glutamine gamma -glutamyltransferase Z, TGase Z and TGZ, is an 80 kDa, widely expressed member of the Transglutaminase superfamily and family. Human Transglutaminase 7 is 710 amino acids (aa) in length and contains a Transglutaminase family, C-terminal Ig like domain (aa 608‑705). Human Transglutaminase 7 shares 78% aa identity with mouse Transglutaminase 7. Like other Transglutaminases, Transglutaminase 7 is an enzyme that stabilizes protein assembly through the formation of intra- or intermolecular N epsilon ( gamma -glutamyl)lysine bonds. It catalyzes a calcium-dependent transferase reaction between the gamma -carboxamide group of a peptide-bound glutamine residue and various primary amines, most commonly the epsilon -amino group of lysine residues.

Alternate Names

TGM7, TGMZ, TGZ

Entrez Gene IDs

116179 (Human); 640543 (Mouse); 691932 (Rat)

Gene Symbol

TGM7

UniProt

Q96PF1

Additional Transglutaminase 7/TGM7 Products

Product Documents for Human Transglutaminase 7/TGM7 Antibody (602708)

Certificate of Analysis

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Product Specific Notices for Human Transglutaminase 7/TGM7 Antibody (602708)

For research use only

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