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Human TRIF/TICAM1 Antibody

Catalog #: AF6216
3 Citations Datasheet / COA / SDS
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AF6216
AF6216-SP

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Detection of Human TRIF/TICAM1 by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRIF/TICAM1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRIF/TICAM1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TRIF/TICAM1
Lys29-Ala204
Accession # Q8IUC6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
25 µg/mL
Raji human Burkitt's lymphoma cell line
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRIF/TICAM1 antibody by Western Blot. View Larger

Detection of Human TRIF/TICAM1 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.25 µg/mL of Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRIF/TICAM1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry TRIF/TICAM1 antibody in Raji Human Cell Line by Immunocytochemistry (ICC). View Larger

TRIF/TICAM1 in Raji Human Cell Line. TRIF/TICAM1 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western View Larger

Detection of Human TRIF/TICAM1 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TRIF/TICAM1 at approximately 122 kDa (as indicated) using 25 µg/mL of Goat Anti-Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIF/TICAM1

TRIF (TIR domain-containing adaptor inducing IFN-beta ; also TICAM1) is a 105-110 kDa cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF 3, -4, and -7, as well as NF kappa B and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712 amino acids (aa) in length and contains three TRAF6 bonding motifs (aa 84-91, 248-255 and 299-309), one TIR domain (aa 390-460), a Pro-rich region (aa 614-678), and an overlapping RHIM domain (aa 661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23 aa substitution for aa 31-162 accompanied by a Pro substitution for aa 633-660, a second shows a 44 aa substitution for aa 352-712, and a third shows a 38 aa substitution for aa 271-712. Over aa 29-204, human TRIF shares 62% aa identity with mouse TRIF.

Long Name
Toll-like receptor adaptor molecule 1
Entrez Gene IDs
148022 (Human); 106759 (Mouse)
Alternate Names
MGC35334; Proline-rich, vinculin and TIR domain-containing protein B; PRVTIRBTIR domain-containing adapter protein inducing IFN-beta; Putative NF-kappa-B-activating protein 502H; TICAM1; TICAM-1TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adaptor molecule 1; toll-like receptor adaptor molecule 1; TRIF; TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta

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Citations for Human TRIF/TICAM1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1beta and TRIF/IRF3.
    Authors: Endo Y, Blinova K, Romantseva T, Golding H, Zaitseva M
    PLoS ONE, 2014-05-28;9(5):e98517.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Enterovirus 68 3C protease cleaves TRIF to attenuate antiviral responses mediated by Toll-like receptor 3.
    Authors: Xiang Z, Li L, Lei X, Zhou H, Zhou Z, He B, Wang J
    J Virol, 2014-03-26;88(12):6650-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. TRIM38 Negatively Regulates TLR3-Mediated IFN-beta Signaling by Targeting TRIF for Degradation.
    Authors: Xue Q, Zhou Z, Lei X, Liu X, He B, Wang J, Hung T
    PLoS ONE, 2012-10-08;7(10):e46825.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot

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