Human TSG-6 Antibody

R&D Systems | Catalog # AF2104

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TSG-6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TSG-6
Trp18-Leu277
Accession # P98066

Specificity

Detects human TSG-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 70% cross-reactivity with recombinant mouse TSG-6 is observed and less than 2% cross-reactivity with recombinant human TSG-14 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TSG-6 Antibody

TSG-6 antibody in human PBMCs by Immunocytochemistry (ICC).

TSG‑6 in human PBMCs.

TSG-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TSG-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2104) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TSG-6 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Western Blot

0.1 µg/mL
Sample: Recombinant Human TSG‑6 (Catalog # 2104-TS)

Reviewed Applications

Read 2 reviews rated 4.5 using AF2104 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSG-6

TSG-6 (TNF-stimulated Gene 6), also known as TNFIP6 is a secreted, 35‑39 kDa group A member of the LINK-Module superfamily of proteins (1‑4). Human TSG-6 is synthesized as a 277 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 260 aa mature region (5, 6). The mature region shows an N-terminal LINK module (amino acids 36‑129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (amino acids 135‑247). Link modules are alpha -helical, beta ‑sheet structures that bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature human TSG-6 shares 94% aa identity with both mouse and canine TSG-6. Cells reported to express TSG-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral, plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or link, between the individual subunits of extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80‑85 kDa HC subunit from I alpha I (inter‑ alpha ‑inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a cation-dependent catalyst for the removal, transfer, and subsequent covalent linkage of HC 1/2 to surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10‑12). The disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

References

  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  6. Lee, T.H. et al. (1992) J. Cell Biol. 116:545.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.

Long Name

Tumor Necrosis Factor-stimulated Gene Sequence 6

Alternate Names

TNFAIP6, TSG6

Entrez Gene IDs

7130 (Human); 21930 (Mouse)

Gene Symbol

TNFAIP6

UniProt

P98066

Additional TSG-6 Products

Product Documents for Human TSG-6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TSG-6 Antibody

For research use only

Citations for Human TSG-6 Antibody

Customer Reviews for Human TSG-6 Antibody (2)

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2 Customer Ratings
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  • Human TSG-6 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: msc
    Species: Human
    Verified Customer | Posted 09/29/2022
    Human TSG-6 Antibody AF2104
  • Human TSG-6 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 01/19/2019
    ICC on UMUC with 1 control and 2 conditions
    Human TSG-6 Antibody AF2104

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