Human UBE2I/Ubc9 Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB11185AFP647

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1050001
View all UBE2I/Ubc9 Primary Antibodies »

Product Specifications

Specificity

Detects Human UBE2I/Ubc9 in direct Elisa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human UBE2I/Ubc9 Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: UBE2I/Ubc9

Ubiquitin-conjugating Enzyme E2I (UBE2I), also known as Ubiquitin-conjugating Enzyme 9 (Ubc9), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. Human UBE2I/Ubc9 shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2I/Ubc9 catalyzes the addition of the Ubiquitin-like protein SUMO to target proteins (1,2). SUMO is transferred from a Ubiquitin-like activating (E1) enzyme heterodimer consisting of SAE1 and UBA2 to Cys93 of UBE2I/Ubc9 (3). In contrast to most Ubiquitin-conjugating (E2) enzymes that function in a complex with Ubiquitin ligases (E3s), UBE2I/Ubc9 can interact directly with protein substrates and may also play a role in substrate recognition (4). UBE2I/Ubc9 mediates the SUMOylation of a variety of proteins including RanGAP1, HDAC4, and PML (5,6). Post-translational modifications of UBE2I/Ubc9, such as auto-SUMOylation at Lys14 or Ser71 phosphorylation by CDC2/CDK1, alter UBE2I/Ubc9 catalytic activity and target protein recognition (5,7). UBE2I/Ubc9-dependent SUMOylation reduces the levels of the stem cell marker Nanog, implicating it in embryonic stem cell pluripotency maintenance (8). SUMOylation also regulates the protein levels of tumor suppressors and oncogenes, and UBE2I/Ubc9 dysregulation is thought to contribute to the pathogenesis of human cancers (9).

References

  1. Desterro, J.M. et al. (1999) J. Biol. Chem. 274:10618.
  2. Johnson, E.S. & G. Blobel (1997) J. Biol. Chem. 272:26799.
  3. Johnson, E.S. et al. (1997) EMBO J. 16:5509.
  4. Yunus, A.A. & C.D. Lima (2006) Nat. Struct. Mol. Biol. 13:491.
  5. Knipscheer, P. et al. (2008) Mol. Cell 31:371.
  6. Duprez, E. et al. (1999) J. Cell Sci. 112:381.
  7. Su, Y.F. et al. (2012) PLoS One 7:e34250.
  8. Wu, Y. et al. (2012) PLoS One 7:e39606.
  9. Mo, Y.Y. et al. (2005) Oncogene 24:2677.

Long Name

Ubiquitin-conjugating Enzyme E2I

Alternate Names

p18, SUMO-protein Ligase, Ubc9, UBCE9

Entrez Gene IDs

7329 (Human); 22196 (Mouse)

Gene Symbol

UBE2I

UniProt

P63279 (Human)

Additional UBE2I/Ubc9 Products

Product Documents for Human UBE2I/Ubc9 Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human UBE2I/Ubc9 Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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