Human VEGF/PlGF Heterodimer Antibody

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Human VEGF/PlGF Heterodimer Antibody Summary

Species Reactivity
Detects human VEGF/PlGF heterodimer in direct ELISAs and Western blots. This antibody does not detect either subunit alone. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF121, rhVEGF165, rhVEGF206, rhCTGF, rhLDGF, rhPlGF, rhVEGF‑B186, rhVEGF‑C, rhVEGF‑D, rhPDGF, rmVEGF115, rmVEGF120, rmVEGF164, rmPlGF‑2, rrVEGF164, or rzfVEGF.
Monoclonal Mouse IgG2A Clone # 146213
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human VEGF/PlGF heterodimer
Ala27-Arg191 (VEGF) & Ala21-Arg149 (PIGF)
Accession # AAM03108 (VEGF) & AAD30179 (PIGF)
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human VEGF/PlGF Heterodimer (Catalog # 297-VP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF/PlGF Heterodimer

The disulfide-linked homodimeric proteins vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) are both members of the VEGF family of growth factors. VEGF and PlGF proteins share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. E. coli-expressed monomers of PlGF and VEGF can be refolded in vitro to form PlGF/VEGF heterodimers. The purified recombinant VEGF/PlGF heterodimers and VEGF homodimers have potent mitogenic effects on endothelial cells. However, VEGF/PlGF heterodimers display approximately 50-fold less mitogenic activity than VEGF homodimers. In contrast, PlGF homodimers have little or no mitogenic effect on endothelial cells. The presence of natural VEGF/PlGF heterodimers in the conditioned media of a number of human tumor cell lines has been demonstrated using a VEGF/PlGF heterodimer specific ELISA.

Long Name
Vascular Endothelial Growth Factor/Placenta Growth Factor Heterodimer
Alternate Names
VEGF/PlGF Heterodimer

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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