IgG2a Kappa Antibody (RM107)
Novus Biologicals | Catalog # NBP2-62007
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM107 expressed in HEK293
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Product Specifications
Immunogen
Mouse IgG
Specificity
This antibody reacts to the Fab region of mouse IgG2a Kappa. No cross reactivity with mouse IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG2a Kappa Antibody (RM107)
Western Blot: IgG2a Kappa Antibody (RM107) [Unconjugated] [NBP2-62007] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a kappa, using NBP2-62007. This antibody only reacts to nonreduced Mouse IgG2a kappa.
ELISA: IgG2a Kappa Antibody (RM107) - Azide and BSA Free [NBP2-62007] -
ELISA: IgG2a Kappa Antibody (RM107) - Azide and BSA Free [NBP2-62007] - A titer ELISA of mouse IgG2a kappa. The plate was coated with different amounts of mouse IgG2a kappa. A serial dilution of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.ELISA: IgG2a Kappa Antibody (RM107) - Azide and BSA Free [NBP2-62007] -
ELISA: IgG2a Kappa Antibody (RM107) - Azide and BSA Free [NBP2-62007] - ELISA of mouse immunoglobulins shows RM107 reacts to the Fab region of mouse IgG2 kappa. No cross reactivity with IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of NBP3-25951 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Applications for IgG2a Kappa Antibody (RM107)
Application
Recommended Usage
ELISA
0.005 ug/ml - 0.2 ug/ml
Western Blot
0.1 ug/ml-0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Product Documents for IgG2a Kappa Antibody (RM107)
Certificate of Analysis
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Product Specific Notices for IgG2a Kappa Antibody (RM107)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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