IgG2a Kappa Antibody (RM107) [Biotin]

Novus Biologicals | Catalog # NBP2-62007B

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Immunocytochemistry

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM107 expressed in HEK293
View all IgG2a Kappa Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

This antibody reacts to the Fab region of mouse IgG2a Kappa. No cross reactivity with mouse IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG2a Kappa Antibody (RM107) [Biotin]

Western Blot: IgG2a Kappa Antibody (RM107) [Biotin] [NBP2-62007B] -

Western Blot: IgG2a Kappa Antibody (RM107) [Biotin] [NBP2-62007B] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a(kappa) (20 ng/lane), using 0.2 ug/mL of NBP2-62007, the unconjugated version of this antibody. This antibody only reacts to nonreduced Mouse IgG2a(kappa).
IgG2a Kappa Antibody (RM107) [Biotin]

ELISA: IgG2a Kappa Antibody (RM107) [Biotin] [NBP2-62007B] -

ELISA: IgG2a Kappa Antibody (RM107) [Biotin] [NBP2-62007B] - ELISA of mouse immunoglobulins shows NBP2-62007B reacts to the Fab region of mouse IgG2ak; no cross reactivity with IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins. NBP2-62007B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG2a Kappa Antibody (RM107) [Biotin]

ELISA: IgG2a Kappa Antibody (RM107) [Biotin] [NBP2-62007B] -

ELISA: IgG2a Kappa Antibody (RM107) [Biotin] [NBP2-62007B] - A titer ELISA of mouse IgG2a kappa. The plate was coated with different amounts of mouse IgG2a kappa. A serial dilution of NBP2-62007B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG2a Kappa Antibody (RM107) [Biotin]

Application
Recommended Usage

ELISA

0.005 ug/ml - 0.2 ug/ml

Immunocytochemistry

0.5 - 2 ug/mL

Western Blot

0.1 ug/ml - 0.5 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Product Documents for IgG2a Kappa Antibody (RM107) [Biotin]

Certificate of Analysis

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Product Specific Notices for IgG2a Kappa Antibody (RM107) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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