IKK alpha Antibody (23GB765)

Name: IKK alpha Antibody (23GB765)
Brand: Novus Biologicals
SKU: NBP3-51084
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-51084

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB765

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Product Specifications

Immunogen

A synthesized peptide derived from human IKK alpha (Uniprot: O15111)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK alpha Antibody (23GB765)

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (23GB765)[NBP3-51084] -

IKK alpha antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: IKK alpha shRNA knockdown (KD). Nuclei were stained blue with DAPI; IKKalpha was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (23GB765)[NBP3-51084] -

Immunocytochemical staining of HepG2 cells with IKK alpha antibody (1:1,000). Nuclei were stained blue with DAPI; IKK alpha was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Western Blot: IKK alpha Antibody (23GB765)[NBP3-51084] -

Western blotting analysis using IKK alpha antibody. IKK alphaexpression in wild type (WT) and IKK alphashRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. Hsp90 alpha serves as a loading control. The blot was incubated with IKK alpha antibody(1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Western Blot: IKK alpha Antibody (23GB765)[NBP3-51084] -

Western blotting analysis using IKK alpha antibody. Total cell lysates (30 ug) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKK alpha antibody(1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (1:50,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

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Applications for IKK alpha Antibody (23GB765)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IKK alpha

IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).

Long Name

IkB Kinase alpha

Alternate Names

CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16

Gene Symbol

CHUK

Additional IKK alpha Products

Product Documents for IKK alpha Antibody (23GB765)

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Product Specific Notices for IKK alpha Antibody (23GB765)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.