Q: Does viral IL-10 cross-react in bioassay?

A: In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.

IL-10 Antibody (JES3-9D7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-77379

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Rhesus Macaque, Virus

Applications

Western Blot, ELISA, ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ kappa Clone # JES3-9D7

Format

Azide and BSA Free

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Product Specifications

Immunogen

COS-expressed human IL-10

Reactivity Notes

Reacts with sooty mangabey (Cercocebus atys).

Specificity

Human/Viral/Rhesus/Cynomolgus/Sooty Mangabey IL-10

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁ kappa

Scientific Data Images for IL-10 Antibody (JES3-9D7) - Azide and BSA Free

ELISA: IL-10 Antibody (JES3-9D7) - Azide and BSA Free [NBP2-77379]

ELISA: IL-10 Antibody (JES3-9D7) [NBP2-77379] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT ( Clone JES3-12G8) followed by Streptavidin-HRP.

Applications for IL-10 Antibody (JES3-9D7) - Azide and BSA Free

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

May be paired with NBP2-27577 in a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

1.0 mL of borate buffered saline, pH 8.2.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IL-10

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for IL-10 Antibody (JES3-9D7) - Azide and BSA Free

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Product Specific Notices for IL-10 Antibody (JES3-9D7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.