JunD Antibody (5L7Y3)

Name: JunD Antibody (5L7Y3)
Brand: Novus Biologicals
SKU: NBP3-16600
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16600

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5L7Y3 expressed in HEK293

View all JunD Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human JunD (P17535). SPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFVKALEDLHKQNQLGAGAAAAAAAAAAGGPSGTATGSAPPGELAPAAAAPEAPVYANLSSY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JunD Antibody (5L7Y3)

Western Blot: JunD Antibody (5L7Y3) [NBP3-16600]

Western Blot: JunD Antibody (5L7Y3) [NBP3-16600] - Western blot analysis of extracts of various cell lines, using JunD Rabbit mAb (NBP3-16600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Chromatin Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using JunD antibody (NBP3-16600) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600]

Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug JunD antibody (NBP3-16600). Western blot was performed from the immunoprecipitate using JunD antibody (NBP3-16600) at a dilition of 1:1000.

Chromatin Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600] -

Chromatin Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using JunD antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

View 1 more images

Applications for JunD Antibody (5L7Y3)

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500 - 1:1000

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JunD

The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

Long Name

JunD Proto-oncogene

Alternate Names

AP-1

Gene Symbol

JUND

Additional JunD Products

Product Documents for JunD Antibody (5L7Y3)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for JunD Antibody (5L7Y3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.