Luciferase Antibody - BSA Free

Novus Biologicals | Catalog # NB110-17348

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Key Product Details

Species Reactivity

Firefly

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Luciferase Primary Antibodies »

Product Specifications

Immunogen

This Luciferase Antibody was developed against full length native protein (purified) (Firefly (Photinus pyralis)).

Localization

Peroxisomal

Specificity

This Luciferase Antibody identifies recombinant luciferase in eukaryotic cells transfected with a plasmid bearing the luciferase gene.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Luciferase Antibody - BSA Free

Western Blot: Luciferase Antibody [NB110-17348]

Western Blot: Luciferase Antibody [NB110-17348]

Western Blot: Luciferase Antibody [NB110-17348] - Lysates of HEK-293T cells overexpressing luciferase was separated on SDS-PAGE and probed with Anti-Luciferase The antibody was developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate. Lanes 1. Antibody dilution 1:1,000 2. Antibody dilution 1:1,000 + luciferase immunizing peptide
Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NB110-17348]

Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NB110-17348]

Immunocytochemistry/Immunofluorescence: Luciferase Antibody [NB110-17348] - HEK-293T cells overexpressing luciferase were fixed and stained 10 ug/mL Anti-Luciferase. The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate.

Applications for Luciferase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Western Blot

1:10000
Application Notes
ICC/IF: A working dilution of 1:5001:1,000 is obtained on methanol-acetone fixed transfected cells using an FITC conjugated secondary antibody. Cells were transfected with a reporter plasmid containing the gene for luciferase.

Reviewed Applications

Read 1 review rated 1 using NB110-17348 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Luciferase

Luciferase is a generic term for a group of oxidative enzymes used in bioluminescence. Firefly (Photinus pyralis) and bacterial luciferase enzymes are commonly used in assay systems such as cell viability assays, reporter gene assays, and for in vivo imaging. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly luciferase is a single polypeptide specified by the luc gene (1). Firefly luciferase (theoretical molecular weight: 61 kDa) oxidizes the substrate luciferin to oxyluciferin in a bioluminescent reaction requiring Mg2+ and ATP (2,3). This reaction produces a flash of yellow-green light with an emission peak around 560nm that can be detected by a luminometer (3). Firefly luciferase has become one of the more widely used reporter proteins and is an excellent tool for the study of gene expression, given that the amount of light emitted is directly proportional to luciferase activity (4).

The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.

References

1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9

2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005

3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b

4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036

Alternate Names

LuC, luciferin 4 monooxygenase, Luciferin 4-monooxygenase

Additional Luciferase Products

Product Documents for Luciferase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Luciferase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Luciferase Antibody - BSA Free (1)

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Customer Images

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  • Luciferase Antibody
    Name: Anonymous
    Application: Immunofluorescence-Frozen
    Sample Tested: Mouse spleen, mouse thymus and Thymus tissue
    Species: Mouse
    Verified Customer | Posted 01/31/2024
    Top: Luc+ mouse thymus stained with anti-luc 1:1K or 1:5K followed by incubation with anti-rabbit 488 L or 555 R. Bottom: WT mouse thymus stained with anti-luc 1:1K or 1:5K followed by incubation with anti-rabbit 488 L or 555 R.
    Frozen Sections were fixed for 10 min in 4% PFA at room temp followed by incubation in 100% Methanol at -20 C for 10 min. Sections were washed and blocked using 10% Donkey Serum or 2% Fish Gelatin/10% Donkey Serum. Tested Primary Dilutions: 1:500, 1:1000, and 1:5000 overnight at 4 C. Secondary Tested: Preabsorbed Anti-Rabbit 488 or Rabbit 555. Tissue collected from transgenic mouse expressing luciferase from CAG promoter. Luciferase activity was confirmed by measuring luminescence from collected tissue before embedding in OCT.
    Luciferase Antibody - BSA Free NB110-17348
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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