MBD1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56330

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MBD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 98-113 (RQVGPQSGEVRKEAPR) and 391-405 (SESEDGAGSPPPYRR) of human MBD1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MBD1 Antibody - BSA Free

Knockdown Validated: MBD1 Antibody [NB100-56330]

Western Blot: MBD1 Antibody [NB100-56330]

Western Blot: MBD1 Antibody [NB100-56330] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human MBD1 Polyclonal Antibody (Catalog # NB100-56330) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: MBD1 Antibody [NB100-56330]

Western Blot: MBD1 Antibody [NB100-56330]

Western Blot: MBD1 Antibody [NB100-56330] - Analysis of MBD1 in HeLa cell lysate with this antibody. A protein band with an approximate molecular weight of 55 kDa was detected.

Applications for MBD1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBD1

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).

Alternate Names

CXXC-type zinc finger protein 3, methyl-CpG binding domain protein 1, methyl-CpG binding domain protein 1 isoform PCM1, methyl-CpG-binding domain protein 1, Methyl-CpG-binding protein MBD1, PCM1CXXC3RFT, Protein containing methyl-CpG-binding domain 1, the regulator of fibroblast growth factor 2 (FGF-2) transcription

Gene Symbol

MBD1

UniProt

NP_056669.2 (Human)

Additional MBD1 Products

Product Documents for MBD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MBD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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