mCherry Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-05558
![Western Blot: mCherry Antibody [NBP3-05558]](https://beta-resources.rndsystems.com/images/products/mCherry-Antibody-Western-Blot-NBP3-05558-img0002.jpg "Western Blot: mCherry Antibody [NBP3-05558]")
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Key Product Details
Species Reactivity
Non-species specific
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat
Format
BSA Free
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Product Specifications
Immunogen
This mCherry Antibody was developed against full length recombinant mCherry protein
Clonality
Polyclonal
Host
Goat
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Goat mCherry Antibody - BSA Free (NBP3-05558) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-mCherry Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for mCherry Antibody - BSA Free
Western Blot: mCherry Antibody [NBP3-05558]
Western Blot: mCherry Antibody [NBP3-05558] - Western blot analysis of HEK293 cell lysates using mCherry Antibody at a dilution of 1:2,000, in green [1] protein standard, [2] HEK293 control cells, [3] HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HA construct. Major band at about 30kDa corresponds to mCherry protein.![Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP3-05558]](https://beta-resources.rndsystems.com/images/products/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05558-img0001.jpg "Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP3-05558]")
Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP3-05558]
Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP3-05558] - Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained with mCherry Antibody at a dilution of 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The mCherry antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, as a result only their nuclei are visible.Applications for mCherry Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% PBS, 50% glycerol
Format
BSA Free
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: mCherry
mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).
References
1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819
2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83
3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992
4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026
5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116
Long Name
mCherry
Alternate Names
DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein
Additional mCherry Products
Product Documents for mCherry Antibody - BSA Free
Product Specific Notices for mCherry Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for mCherry Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for mCherry Antibody - BSA Free
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Q: Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
A: mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
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