MKL2 Antibody (CL1546) - BSA Free
Novus Biologicals | Catalog # NBP2-52970
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL1546
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q9ULH7, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MKL2 Antibody (CL1546) - BSA Free
Western Blot: MKL2 Antibody (CL1546) [NBP2-52970]
Western Blot: MKL2 Antibody (CL1546) [NBP2-52970] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4Immunocytochemistry/ Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970]
Immunocytochemistry/Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970] - Staining of A549 cells using the Anti-MKL2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).Immunocytochemistry/ Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970]
Immunocytochemistry/Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970] - Staining of A549 cells using the Anti-MKL2 monoclonal antibody, showing specific staining in nuclear speckles and the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.Applications for MKL2 Antibody (CL1546) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MKL2
Alternate Names
DKFZp686J1745, FLJ31823, FLJ45623, KIAA1243, megakaryoblastic leukemia 2, MKL/myocardin-like 2, MKL/myocardin-like protein 2, MRTFB, MRTF-B, myocardin-related transcription factor B, NPD001
Gene Symbol
MRTFB
Additional MKL2 Products
Product Documents for MKL2 Antibody (CL1546) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MKL2 Antibody (CL1546) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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