MNK2 Antibody (2A10) - Azide and BSA Free

Novus Biologicals | Catalog # H00002872-M07

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2A10

Format

Azide and BSA Free
View all MNK2 Primary Antibodies »

Product Specifications

Immunogen

MKNK2 (AAH18345, 1 a.a. ~ 158 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRWDSHFLLPPHPCRIHVRPGGLVRTVTVNE

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

MKNK2 (2A10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MNK2 Antibody (2A10) - Azide and BSA Free

Western Blot: MNK2 Antibody (2A10) [H00002872-M07]

Western Blot: MNK2 Antibody (2A10) [H00002872-M07]

Western Blot: MNK2 Antibody (2A10) [H00002872-M07] - Analysis of MKNK2 expression in transfected 293T cell line by MKNK2 monoclonal antibody (M07), clone 2A10. Lane 1: MKNK2 transfected lysatE (46.7 KDa). Lane 2: Non-transfected lysate.
Western Blot: MNK2 Antibody (2A10) [H00002872-M07]

Western Blot: MNK2 Antibody (2A10) [H00002872-M07]

Western Blot: MNK2 Antibody (2A10) [H00002872-M07] - Analysis of MKNK2 expression in PC-12 (Cat # L012V1).
Western Blot: MNK2 Antibody (2A10) [H00002872-M07]

Western Blot: MNK2 Antibody (2A10) [H00002872-M07]

Western Blot: MNK2 Antibody (2A10) [H00002872-M07] - Analysis of MKNK2 expression in NIH/3T3 (Cat # L018V1).
Western Blot: MNK2 Antibody (2A10) [H00002872-M07]

Western Blot: MNK2 Antibody (2A10) [H00002872-M07]

Western Blot: MNK2 Antibody (2A10) [H00002872-M07] - Analysis of MKNK2 expression in K-562 (Cat # L009V1).
ELISA: MNK2 Antibody (2A10) [H00002872-M07]

ELISA: MNK2 Antibody (2A10) [H00002872-M07]

ELISA: MNK2 Antibody (2A10) [H00002872-M07] - Detection limit for recombinant GST tagged MKNK2 is approximately 0.3ng/ml as a capture antibody.
Proximity Ligation Assay: MNK2 Antibody (2A10) [H00002872-M07]

Proximity Ligation Assay: MNK2 Antibody (2A10) [H00002872-M07]

Proximity Ligation Assay: MNK2 Antibody (2A10) [H00002872-M07] - Analysis of protein-protein interactions between MAPK14 and MKNK2 HeLa cells. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for MNK2 Antibody (2A10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MNK2

MNK2 may play a role in the response to environmental stress and cytokines. Appears to regulate transcription byphosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap

Long Name

MAP kinase-interacting serine/threonine-protein kinase 2

Alternate Names

MKNK2

Entrez Gene IDs

2872 (Human)

Gene Symbol

MKNK2

OMIM

605069 (Human)

UniProt

AAH18345 (Human)

Additional MNK2 Products

Product Documents for MNK2 Antibody (2A10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MNK2 Antibody (2A10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MNK2 Antibody (2A10) - Azide and BSA Free

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