Key Product Details

Species Reactivity

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all B7-H2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
Glu47-Lys279
Accession # Q9JHJ8

Specificity

Detects mouse B7‑H2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse B7-H2 Antibody

B7‑H2 in Mouse Splenocytes.

B7‑H2 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse B7‑H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF158) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse B7-H2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse B7-H2 Fc Chimera (Catalog # 158-B7)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: B7-H2

Mouse B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. The extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20‑25% amino acid identity. Mouse and human B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Mouse B7-H2 is a 322 amino acid (aa) protein with a putative 46 aa signal peptide, a 233 aa extracellular domain, a 19 aa transmembrane region, and a 24 aa cytoplasmic domain. Mouse B7-H2 is expressed constitutively on resting B cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References

  1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  2. Ling, V. et al. (2000) J. Immunol. 164:1653.
  3. Wang, S. et al. (2000) Blood 96:2808.
  4. Brodie, D. et al. (2000) Curr. Biol. 10:333.
  5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  6. Swallow, M.M. et al. (1999) Immunity 11:423.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Entrez Gene IDs

23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)

Gene Symbol

ICOSLG

UniProt

Q9JHJ8

Additional B7-H2 Products

Product Documents for Mouse B7-H2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse B7-H2 Antibody

For research use only

Citations for Mouse B7-H2 Antibody

Customer Reviews for Mouse B7-H2 Antibody

There are currently no reviews for this product. Be the first to review Mouse B7-H2 Antibody and earn rewards!

Have you used Mouse B7-H2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies