Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 719040
View all BMP-6 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse BMP-6
Met1-His510
Accession # P20722

Specificity

Detects mouse BMP-6 in ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse BMP‑6 Antibody

Alkaline Phosphatase Production Induced by BMP-6 and Neutralization by Mouse BMP-6 Antibody.

Alkaline Phosphatase Production Induced by BMP-6 and Neutralization by Mouse BMP-6 Antibody.

Recombinant Mouse BMP-6 (Catalog # 6325-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP-6 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP-6 Monoclonal Antibody (Catalog # MAB6325). The ND50 is typically 1.5-7.5 µg/mL.

Applications for Mouse BMP‑6 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize BMP‑6-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Asahina, I. et al. (1996) Exp. Cell Res. 222:38. The Neutralization Dose (ND50) is typically 1.5-7.5 μg/mL in the presence of 0.5 μg/mL Recombinant Mouse BMP‑6.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-6

Bone Morphogenetic Protein 6 (BMP‑6), also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins (1, 2). Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3‑5). Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin (7, 8). BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (5), chondrocyte maturation (9), Ang II-induced aldosterone production in the adrenal cortex (3), hormone production and responsiveness in ovarian granulosa cells (10), iNOS and TNF-alpha production in macrophages (4), the cell death of B cells (8), and neurite outgrowth (11). BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone (13). Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (14, 15).

References

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Lyons, K. et al. (1989) Proc. Natl. Acad. Sci. 86:4554.
  3. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  4. Hong, J.H. et al. (2008) Immunology 128:e442.
  5. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  6. Saremba, S. et al. (2008) FEBS J. 275:172.
  7. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  8. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  9. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  10. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Meynard, D. et al. (2009) Nat. Genet. 41:478.
  15. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

Long Name

Bone Morphogenetic Protein 6

Alternate Names

BMP6, Vgr-1

Entrez Gene IDs

654 (Human); 12161 (Mouse); 25644 (Rat)

Gene Symbol

BMP6

UniProt

P20722 (Human)

Additional BMP-6 Products

Product Documents for Mouse BMP‑6 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse BMP‑6 Antibody

For research use only

Citations for Mouse BMP‑6 Antibody

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