Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL22/MDC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # Q9QZU2

Specificity

Detects mouse CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CCL22/MDC is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL22/MDC Antibody

Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody.

Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody.

Recombinant Mouse CCL22/MDC (Catalog # 439-MD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL22/MDC (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439). The ND50 is typically 0.3-1.0 µg/mL.
Detection of CCL22/MDC by Immunohistochemistry

Detection of CCL22/MDC by Immunohistochemistry

G‐CSF and MIP‐2 in exosomes were the main protection effectors in testis radiation protection. A, On day 7 after neutralizing antibody administration and 4 Gy irradiation, mice testis was isolated and subjected to H&E staining. B, Clonal formation assay was used to determine GC‐1 cell viability after exosomes treatment and irradiation exposure with or without neutralizing antibody. Data were presented as mean ± SD (n = 3). *P < .05. **P < .01, ***P < .001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32135028), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCL22/MDC Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL22/MDC (Catalog # 439-MD)

Neutralization

Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse CCL22/MDC.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL22/MDC

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

References

  1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
  2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
  3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Alternate Names

ABCD-1, MDC, STCP-1

Entrez Gene IDs

6367 (Human); 20299 (Mouse)

Gene Symbol

CCL22

UniProt

Q9QZU2

Additional CCL22/MDC Products

Product Documents for Mouse CCL22/MDC Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CCL22/MDC Antibody

For research use only

Citations for Mouse CCL22/MDC Antibody

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