Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) precursor protein with a 22 aa signal peptide and a 108 aa mature protein. Mature human and mouse CCL28 share 83% aa identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10, which is also the receptor for CCL27/CTACK.
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Key Product Details
Species Reactivity
Mouse
Applications
ELISA Capture (Matched Antibody Pair)
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 134315
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Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2
Ile23-Arg130
Accession # Q9JIL2
Specificity
Detects mouse CCL28 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human CCL28 or recombinant mouse CCL27 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CCL28 Antibody (134315)
Mouse CCL28 ELISA Standard Curve
Recombinant Mouse CCL28 (Catalog # 533-VI) was serially diluted and captured by Rat Anti-Mouse CCL28 Monoclonal Antibody (Catalog # MAB5331) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF533) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse CCL28 Antibody (134315)
Mouse CCL28 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL28
References
- Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Alternate Names
CCL28, MEC, VIC
Gene Symbol
CCL28
UniProt
Additional CCL28 Products
Product Documents for Mouse CCL28 Antibody (134315)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CCL28 Antibody (134315)
For research use only
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Associated Pathways
