CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3, and 5.
Mouse CCL5/RANTES Antibody (53433)
R&D Systems | Catalog # MAB4781
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
ELISA Capture, ELISA Development, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 53433
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Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL5/RANTES
Ser24-Ser91
Accession # P30882
Ser24-Ser91
Accession # P30882
Specificity
Detects recombinant mouse (rm)CCL5 direct ELISAs. In sandwich ELISAs, approximately 5% cross-reactivity with rhCCL5 and less than 0.2% cross-reactivity with rhTECK and rhVIC was observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse CCL5/RANTES Antibody (53433)
Mouse CCL5 / RANTES ELISA Standard Curve
Recombinant Mouse CCL5/RANTES (Catalog # 478-MR) was serially diluted and captured by Rat Anti-Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB4781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse CCL5/RANTES Antibody (53433)
Mouse CCL5/RANTES Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL5/RANTES
Alternate Names
RANTES, SISd
Gene Symbol
CCL5
UniProt
Additional CCL5/RANTES Products
Product Documents for Mouse CCL5/RANTES Antibody (53433)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CCL5/RANTES Antibody (53433)
For research use only
Citations for Mouse CCL5/RANTES Antibody (53433)
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Associated Pathways
