Mouse CD90/Thy1 Antibody

Detection of Mouse CD90/Thy1 by Western Blot.

Western blot shows lysates of mouse thymus tissue, mouse brain tissue, EL-4 mouse lymphoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7335) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 25-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of CD90/Thy1 by Immunohistochemistry

In vivo detection of two MSP limb bud subpopulations based on MSC-am localization. (A) Transversal section of an E10.5 mouse embryo to show the histological organization at this stage; the immunodetections of CD29, SCA-1, CD44, CD105, CD90, and CD73 markers in E10.5 limb buds are shown. The limb bud for each MSC-am marker corresponds to the region inside the square in panel (A). (B) Dot plots of the multiparametric flow cytometry analysis showing the hierarchy to identify sSca+ and sSca– subpopulations in E10.5, E11.5, and E12.5 mouse limbs. The number of positive cells for each subpopulation is expressed in percentage; ±mean standard deviation. (C) Comparison of the expression percentage of the sSca+ subpopulation between E10.5, E11.5, and E12.5 limbs. Statistical significance was set as follows: E10.5 vs. E11.5 p > 0.0061; 10.5 vs. 12.5 p > 0.0023; E11.5 vs. E12.5 p > 0.4079, **p < 0.005. NT, neural tube; AGM, aorta-gonad-mesonephros; Do, dorsal; V, ventral; Pr, proximal; D, distal. In (A) AER is marked with an asterisk. The scale bar is set at 200 μm and is representative for all images. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34336823), licensed under a CC-BY license. Not internally tested by R&D Systems.