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Mouse CXCL16 Antibody (142417)

R&D Systems | Catalog # MAB503

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, ELISA Development, In vivo assay, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 142417
View all CXCL16 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL16
Asn27-Pro114
Accession # Q8BSU2

Specificity

Detects mouse CXCL16 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12, 13, 14, 15, recombinant rat CXCL1, 2, 3, 5, or recombinant porcine CXCL8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCL16 Antibody (142417)

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.

Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503). The ND50 is typically 0.15-0.6 µg/mL.
Detection of Mouse CXCL16 by Flow Cytometry

Detection of Mouse CXCL16 by Flow Cytometry

CXCL16 neutralization leads to decreased percentage of TRM cells in the CNS after viral clearance. a. Schematic depicting experimental design for CXCL16 neutralizing an-tibody experiment. Mice were infected (i.c.) with 1×104 p.f.u. WNV-NS5-E218A and administered CXCL16 antibody or isotype control via retro-orbital injection, and harvested 15 DPI. b. Quantifi-cation of percentage of CD45high cells that are CD8+ in the cortex (left) and hippocampus (right) in mice that received isotype or CXCL16 neutralizing antibody. c. Gating strategy and quantifi-cation of percentage of CD8+ T cells that are CD103+ in the cortex and hippocampus in mice that received isotype or CXCL16 neutralizing antibody. Data represent the mean±s.e.m. and were analyzed by unpaired Student’s t-test. *P<0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36153630), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CXCL16 by Flow Cytometry

Detection of Mouse CXCL16 by Flow Cytometry

CXCL16 neutralization leads to decreased percentage of TRM cells in the CNS after viral clearance. a. Schematic depicting experimental design for CXCL16 neutralizing an-tibody experiment. Mice were infected (i.c.) with 1×104 p.f.u. WNV-NS5-E218A and administered CXCL16 antibody or isotype control via retro-orbital injection, and harvested 15 DPI. b. Quantifi-cation of percentage of CD45high cells that are CD8+ in the cortex (left) and hippocampus (right) in mice that received isotype or CXCL16 neutralizing antibody. c. Gating strategy and quantifi-cation of percentage of CD8+ T cells that are CD103+ in the cortex and hippocampus in mice that received isotype or CXCL16 neutralizing antibody. Data represent the mean±s.e.m. and were analyzed by unpaired Student’s t-test. *P<0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36153630), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mouse CXCL16 ELISA Standard Curve

Mouse CXCL16 ELISA Standard Curve

Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) was serially diluted and captured by Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF503) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CXCL16 Antibody (142417)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CXCL16-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nature Immunol. 1:298. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse CXCL16 Chemokine Domain.

Mouse CXCL16 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CXCL16 Biotinylated Antibody (Catalog # BAF503)
  • Standard: Recombinant Mouse CXCL16 Protein (Catalog # 503-CX)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB503 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL16

Mouse CXCL16 (CXC chemokine 16) is a non-ELR motif-containing CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine andCXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa signal peptide, an 88 aa chemokine domain, an 87 aa mucin-like spacer region, a 22 aa transmembrane domain, and a 23 aa cytoplasmic tail. Mouse and human CXCL16 share 49% aa sequence identity. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and bycells in the splenic red pulp both as membrane-bound and soluble forms. Based on northern blot analysis, CXCL16 is also expressed in some non-lymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6 (previously called Bonzo, STRL33 or TYMSTR), which is also a coreceptor for HIV entry. CXCR6 is expressed on naive CD8 cells, natural killer T cells and activated CD8 and CD4 T cells.

Alternate Names

CXCL16

Entrez Gene IDs

58191 (Human); 66102 (Mouse)

Gene Symbol

CXCL16

UniProt

Q8BSU2

Additional CXCL16 Products

Product Documents for Mouse CXCL16 Antibody (142417)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCL16 Antibody (142417)

For research use only

Citations for Mouse CXCL16 Antibody (142417)

Customer Reviews for Mouse CXCL16 Antibody (142417) (1)

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  • Mouse CXCL16 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: BaF3 mouse pro‑B cell line
    Species: Mouse
    Verified Customer | Posted 09/24/2021
    Mouse CXCL16 Antibody (142417) MAB503

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