Mouse DPPA4 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF3730U Datasheet / COA / SDS

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AF3730U-100UG

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Mouse DPPA4 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DPPA4 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human DPPA4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse DPPA4
Asn125-Gly296
Accession # Q8CCG4

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DPPA4

DPPA4 is a 33 kDa molecule that is expressed specifically in the early embryo, uncommitted cells of the germ line, and embryonic stem cells and is used as a marker of pluripotent cells. Mouse DPPA4 is 296 amino acids in length and contains an SAP (SAF-A/B, ACINUS, PIAS) putative DNA-binding domain. Two potential isoforms exist with deletions of amino acids 104‑120 and 69‑120, respectively. In the region used as an immunogen, mouse DPPA4 shows 57% amino acid sequence identity with human and 87% identity with rat DPPA4 proteins.

Long Name

Developmental Pluripotency Associated 4

Entrez Gene IDs

55211 (Human); 73693 (Mouse)

Alternate Names

developmental pluripotency associated 4; developmental pluripotency-associated protein 4; DPPA4; FLJ10713,2410091M23Rik