Mouse DPPA5/ESG1 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF3984S Datasheet / COA / SDS

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AF3984S-100UG

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All DPPA5/ESG1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DPPA5/ESG1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DPPA5/ESG1 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human DPPA5 is observed and approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse DPPA5/ESG1
Met1-Glu118
Accession # Q9CQS7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DPPA5/ESG1

DPPA5, also known as Esg-1 and ECAT2, is a 14 kDa RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state. Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid identity with human and 81% amino acid identity with rat DPPA5 proteins.

Long Name

Developmental Pluripotency Associated 5

Entrez Gene IDs

340168 (Human); 434423 (Mouse); 301101 (Rat)

Alternate Names

developmental pluripotency associated 5; developmental pluripotency-associated 5 protein; DPPA5; embryonal stem cell specific gene 1; Embryonal stem cell-specific gene 1 protein; ESG1; ESG1ESG-1; hDPPA5