Mouse Endosialin/CD248 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB7535U Datasheet / COA / SDS

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FAB7535U-100UG

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Mouse Endosialin/CD248 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Endosialin/CD248 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endosialin/CD248 is observed.

Source

Monoclonal Rat IgG2a Clone # 458606

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endosialin/CD248
Val13-Arg693 (predicted)
Accession # Q91V98

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Endosialin/CD248

Endosialin, also known as CD248 and tumor endothelial marker 1 (Tem1), is a 165 kDa transmembrane O-glycosylated protein that contains one C-type lectin, one Sushi, one EGF-like domain and a mucin-like stalk in its extracellular domain (ECD). It is expressed on activated perivascular and stromal cells in embyronic and tumor neovasculature but is downregulated in quiescent vasculature. Endosialin regulates pericyte proliferation, migration, and adhesion to matrix Fibronectin and Collagens I and IV. It is also required for lymph node and spleen remodeling during immune responses. Within the ECD, mouse Endosialin shares 76% and 93% aa sequence identity with human and rat Endosialin, respectively.

Entrez Gene IDs

57124 (Human); 70445 (Mouse); 293669 (Rat)

Alternate Names

2610111G01Rik; CD164 sialomucin-like 1; CD164L1; CD248 antigen; CD248 antigen, endosialin; CD248 molecule, endosialin; CD248; Endosialin; MGC119479; TEM1; TEM1MGC119478; Tumor endothelial marker 1