Mouse FGFR5/FGFRL1 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB1899S Datasheet / COA / SDS

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FAB1899S-100UG

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All FGFR5/FGFRL1 Antibodies

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Mouse FGFR5/FGFRL1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF R5/FGFRL1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF R1 alpha (IIIb), 1 alpha (IIIc), 1 beta (IIIb), 1 beta (IIIc), recombinant mouse (rm) FGF R2 alpha (IIIb), 2 alpha (IIIc), 2

Source

Monoclonal Rat IgG2b Clone # 223003

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FGF R5/FGFRL1
Ala21-Pro374
Accession # Q91V87

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FGFR5/FGFRL1

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2‑6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6).

Long Name

Fibroblast Growth Factor Receptor-like 1

Entrez Gene IDs

53834 (Human); 116701 (Mouse)

Alternate Names

FGF homologous factor receptor; FGF R5; FGF receptor-like protein 1; FGFR5; FGFR-5; FGFRL1; FGFR-like protein; FHFR; Fibroblast growth factor receptor 5; fibroblast growth factor receptor-like 1