Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Mouse IFN-alpha Antibody (RMMA-1)
R&D Systems | Catalog # 22100-1
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
ELISA, Neutralization
Cited:
Immunohistochemistry, Neutralization, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # RMMA-1
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Product Specifications
Immunogen
Mouse Interferon alpha
Specificity
Neutralizes mouse interferon alpha; binds to mouse interferon alpha
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse IFN-alpha Antibody (RMMA-1)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Bicarbonate; 0.2M Sodium Chloride, pH 8.0
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Re-freezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
References
- Asselin-Paturel, C. et al. 2005, J. Exp. Med. 201:1157.
- Mellor, A.L. et al. 2005, J. Immunol. 175:5601.
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Mouse IFN-alpha Antibody (RMMA-1)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IFN-alpha Antibody (RMMA-1)
For research use only
Citations for Mouse IFN-alpha Antibody (RMMA-1)
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Protocols
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