Mouse IFN-gamma Antibody (37801/37875)

Detection of Mouse IFN-gamma by ELISA

A27L-specific cell-mediated immune response against recombinant VVWR determined by ELISPOT analysis.(A) Each group consisted of four female Balb/C mice immunized three times, two weeks apart via intramuscular injection as follows: Naïve (negative control), pVax1 (empty vector backbone control), pA27L (VVWR envelope protein), pA27L+I (VVWR envelope protein + imiquimod), pA27L + R (VVWR envelope protein + resiquimod), pA27LOPT (optimized sequence of the A27L gene) and pA27LOPT+I (the optimized sequence of A27L + imiquimod). (B) Identification of A27L dominant epitopes. Antigen-specific IFN-gamma ELISPOT in splenocytes from mice (immunized three times, two weeks apart via intramuscular injection) in response to VVWR A27 individual 3-mer overlapped 15-mer overlapping peptides. Each experiment was performed three times and the immune responses among groups of mice are presented as the mean ± standard error of the mean (SEM). A p value of less than 0.05 was considered significant. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25875833), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse IFN-gamma ELISA Standard Curve

Recombinant Mouse IFN‑ gamma (Catalog # 485-MI) was serially diluted and captured by Rat Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB785) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-485-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)