Mouse IL-36Ra/IL-1F5 Antibody (759207)

R&D Systems | Catalog # MAB2714

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 759207
View all IL-36Ra/IL-1F5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-36Ra/IL-1F5
Met1-Cys155
Accession # Q9QYY1

Specificity

Detects mouse IL-36Ra/IL-1F5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human IL-36Ra and recombinant mouse IL-1ra is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse IL-36Ra/IL-1F5 Antibody (759207)

Detection of Mouse IL-36Ra/IL-1F5 antibody by Western Blot.

Detection of Mouse IL‑36Ra/IL‑1F5 by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-36Ra/IL-1F5 Monoclonal Antibody (Catalog # MAB2714) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-36Ra/IL-1F5 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IL-36Ra/IL-1F5 Antibody (759207)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-36Ra/IL-1F5

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1 delta  [delta], IL‑1HY1, IL-1H3, and IL-1L1) is a member of the IL‑1 family of proteins (1-6). IL‑1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL‑18 and IL‑1F5 through F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL‑36Ra/IL‑1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL‑18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL‑36Ra/IL‑1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL‑36Ra/IL‑1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL‑1ra, IL‑1 beta, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL-36 beta /IL-1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). Indirect evidence suggests the receptor for IL-36Ra/IL-1F5 is IL‑1 Rrp2 and/or IL-1 RAcP (9). IL-36Ra/IL-1F5 was initially reported to be an antagonist of  IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL‑1ra.

References

  1. Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
  4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  5. Barton J. L. et al. (2000) Eur. J. Immunol. 30:3299.
  6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  7. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  8. Debets, R. et al. (2001) J. Immunol. 167:1440.
  9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5

Alternate Names

FIL1 delta, IL-1HY1, IL-1L1, IL-1RP3, IL1F5, IL36Ra, IL36RN

Entrez Gene IDs

26525 (Human); 54450 (Mouse)

Gene Symbol

IL36RN

UniProt

Q9QYY1

Additional IL-36Ra/IL-1F5 Products

Product Documents for Mouse IL-36Ra/IL-1F5 Antibody (759207)

Certificate of Analysis

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Product Specific Notices for Mouse IL-36Ra/IL-1F5 Antibody (759207)

For research use only

Related Research Areas

IL-1 Family

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Associated Pathways

IL-1 Family Signaling Pathways
IL-1 Family Signaling Pathway Thumbnail