IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL)
Sensitivity
8.3 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
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Product Summary for Mouse IL-7 Quantikine ELISA Kit
The Quantikine Mouse IL-7 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-7 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-7 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-7 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-7.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse IL-7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
精度
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 41 | 41 | 41 |
| Mean (pg/mL) | 82 | 211 | 819 | 84 | 215 | 770 |
| Standard Deviation | 3.5 | 5.9 | 29.6 | 6.7 | 16.6 | 67.1 |
| CV% | 4.3 | 2.8 | 3.6 | 8.0 | 7.7 | 8.7 |
Recovery for Mouse IL-7 Quantikine ELISA Kit
The recovery of mouse IL-7 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 110 | 107-113 |
| EDTA Plasma (n=4) | 109 | 100-118 |
| Serum (n=4) | 108 | 96-120 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-7 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IL-7 Quantikine ELISA Kit
Mouse IL-7 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-7
Long Name
Interleukin 7
Alternate Names
IL7, Lymphopoietin-1, PBGF
Gene Symbol
IL7
Additional IL-7 Products
Product Documents for Mouse IL-7 Quantikine ELISA Kit
Product Specific Notices for Mouse IL-7 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse IL-7 Quantikine ELISA Kit
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4.9 out of 5
12 Customer Ratings
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Sample Tested: Cell culture supernatantVerified Customer | Posted 10/26/2022cell culture supernatant from transfected BHK-21 cells
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Sample Tested: Cell culture supernatantVerified Customer | Posted 11/19/2021
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Sample Tested: Serum and Lymph node cellsVerified Customer | Posted 05/30/2018
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Sample Tested: Pancreas tissue and PlasmaVerified Customer | Posted 04/13/2018
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Sample Tested: BW5147 mouse T lymphoma cell lineVerified Customer | Posted 04/12/2018
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Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell lineVerified Customer | Posted 03/30/2018
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Sample Tested: E4.5 mouse embryoVerified Customer | Posted 03/14/2018
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Sample Tested: Serum-free Cell Culture SupernatesVerified Customer | Posted 03/08/2018
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Sample Tested: 4T1 mouse breast cancer cell lineVerified Customer | Posted 03/07/2018
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Sample Tested: 4T1 mouse breast cancer cell lineVerified Customer | Posted 02/17/2018
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Sample Tested: Cell culture supernatantVerified Customer | Posted 02/14/2018
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Sample Tested: SerumVerified Customer | Posted 02/08/2018
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Protocols
View specific protocols for Mouse IL-7 Quantikine ELISA Kit (M7000):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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