Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 118125
View all OSMR beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta
Glu24-Leu738
Accession # O70458

Specificity

Detects mouse OSM R beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CLC, rhCNTF, rhOSM, recombinant mouse (rm) CT-1, rmIL-6, rmIL-11, or rmLIF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse OSMR beta Antibody

OSM R beta antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC).

OSM R beta in D3 Mouse Cell Line.

OSM R beta was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse OSM R beta Monoclonal Antibody (Catalog # MAB662) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse OSMR beta Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse OSM R beta Fc Chimera (Catalog # 662-OR)

Reviewed Applications

Read 1 review rated 5 using MAB662 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OSMR beta

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) type I transmembrane protein which contains a 23 aa signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.

References

  1. Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
  2. Tanaka, M. et al. (1999) Blood 93:804.

Long Name

Oncostatin M Receptor beta

Alternate Names

OSM R beta, OSMR, OSMRB

Entrez Gene IDs

9180 (Human); 18414 (Mouse); 310132 (Rat)

Gene Symbol

OSMR

UniProt

O70458

Additional OSMR beta Products

Product Documents for Mouse OSMR beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse OSMR beta Antibody

For research use only

Citations for Mouse OSMR beta Antibody

Customer Reviews for Mouse OSMR beta Antibody (1)

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  • Mouse OSMR beta Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Dendritic cells
    Species: Mouse
    Verified Customer | Posted 01/25/2022
    Mouse OSMR beta Antibody MAB662

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