NDUFB7 Antibody (4D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00004713-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4D4

Format

Azide and BSA Free
View all NDUFB7 Primary Antibodies »

Product Specifications

Immunogen

NDUFB7 (NP_004137, 38 a.a. ~ 137 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EMVATQQEMMDAQLRLQLRDYCAHHLIRLLKCKRDSFPNFLACKQERHDWDYCEHRDYVMRMKEFERERRLLQRKKRREKKAAELAKGQGPGEVDPKVAL

Specificity

NDUFB7 (4D4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NDUFB7 Antibody (4D4) - Azide and BSA Free

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01]

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01]

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - Analysis of NDUFB7 expression in transfected 293T cell line by NDUFB7 monoclonal antibody (M01), clone 4D4.Lane 1: NDUFB7 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01]

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01]

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - Analysis of NDUFB7 over-expressed 293 cell line, cotransfected with NDUFB7 Validated Chimera RNAi ( Cat # H00004713-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFB7 monoclonal antibody (M01), clone 4D4 (Cat # H00004713-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01]

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01]

Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - NDUFB7 monoclonal antibody (M01), clone 4D4 Analysis of NDUFB7 expression in A-431.
ELISA: NDUFB7 Antibody (4D4) [H00004713-M01]

ELISA: NDUFB7 Antibody (4D4) [H00004713-M01]

ELISA: NDUFB7 Antibody (4D4) [H00004713-M01] - Detection limit for recombinant GST tagged NDUFB7 is approximately 1ng/ml as a capture antibody.

Applications for NDUFB7 Antibody (4D4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDUFB7

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternate Names

B18, Cell adhesion protein SQM1, CI-B18NADH-ubiquinone oxidoreductase B18 subunit, complex I B18 subunit, Complex I-B18, MGC2480, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7 (18kD, B18), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Entrez Gene IDs

4713 (Human)

Gene Symbol

NDUFB7

OMIM

603842 (Human)

UniProt

NP_004137 (Human)

Additional NDUFB7 Products

Product Documents for NDUFB7 Antibody (4D4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NDUFB7 Antibody (4D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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