NSD3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33013

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSD3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NSD3 Antibody - BSA Free

NSD3 Antibody

Immunocytochemistry/ Immunofluorescence: NSD3 Antibody [NBP1-33013] -

Immunocytochemistry/ Immunofluorescence: NSD3 Antibody [NBP1-33013] - NSD3 antibody [C1C2], Internal detects NSD3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSD3 protein stained by NSD3 antibody [C1C2], Internal (NBP1-33013) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.
NSD3 Antibody

Western Blot: NSD3 Antibody [NBP1-33013] -

Western Blot: NSD3 Antibody [NBP1-33013] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NSD3 antibody [C1C2], Internal (NBP1-33013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: NSD3 Antibody [NBP1-33013]

Western Blot: NSD3 Antibody [NBP1-33013]

Western Blot: NSD3 Antibody [NBP1-33013] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.

Applications for NSD3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NSD3

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described.

Alternate Names

EC 2.1.1.43, FLJ20353, histone-lysine N-methyltransferase NSD3, MGC126766, MGC142029, NSD3DKFZp667H044, Nuclear SET domain-containing protein 3, pp14328, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, WHSC1-like protein 1, Wolf-Hirschhorn syndrome candidate 1-like 1, Wolf-Hirschhorn syndrome candidate 1-like protein 1

Entrez Gene IDs

54904 (Human)

Gene Symbol

NSD3

UniProt

Additional NSD3 Products

Product Documents for NSD3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NSD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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