NSUN4 Antibody (4G3-R)

Immunocytochemistry/ Immunofluorescence: NSUN4 Antibody (4G3-R) [NBP3-32666]

Immunocytochemistry/ Immunofluorescence: NSUN4 Antibody (4G3-R) [NBP3-32666] - Immunocytochemistry analysis of MCF7 cells labeling NSUN4 with Mouse anti-NSUN4 antibody (NBP3-32666) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NSUN4 antibody (NBP3-32666) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.

Western Blot: NSUN4 Antibody (4G3-R) [NBP3-32666]

Western blot analysis on different lysates with NBP3-32666 at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32666 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.