Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2404B
View all NucA nuclease Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived S. marcescens recombinant NucA nuclease
Met1-Asn266
Accession # P13717

Specificity

Detects NucA nuclease in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NucA nuclease Antibody

NucA nuclease Antibody in ELISA Standard Curve.

NucA nuclease ELISA Standard Curve.

Recombinant NucA nuclease was serially diluted 2-fold and captured by Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB10063) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-NucA nuclease Monoclonal Antibody (MAB100632) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Detection of NucA nuclease®NucA nuclease antibody by Western Blot.

Detection of NucA nuclease by Western Blot.

Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB10063) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of NucA nuclease antibody by Simple WesternTM.

Detection of NucA nuclease by Simple WesternTM.

Simple Western lane view shows lysates of recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB10063). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for NucA nuclease Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100632).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Simple Western

10 µg/mL
Sample: Recombinant NucA nuclease Protein

Western Blot

2 µg/mL
Sample: Recombinant NucA nuclease Protein

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NucA nuclease

Long Name

NucA nuclease

Alternate Names

DcD

Entrez Gene IDs

23387471 (Bacterial)

Gene Symbol

SMDB11_RS05280

UniProt

P13717

Additional NucA nuclease Products

Product Documents for NucA nuclease Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for NucA nuclease Antibody

For research use only

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FAQs

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