ORP8 Antibody - Azide Free
Novus Biologicals | Catalog # NBP2-19634
![Western Blot: ORP8 Antibody [NBP2-19634]](https://resources.rndsystems.com/images/products/ORP8-Antibody-Western-Blot-NBP2-19634-img0001.jpg "Western Blot: ORP8 Antibody [NBP2-19634]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (96%), Rat (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ORP8. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ORP8 Antibody - Azide Free
Western Blot: ORP8 Antibody [NBP2-19634]
Western Blot: ORP8 Antibody [NBP2-19634] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:10000.
Western Blot: ORP8 Antibody [NBP2-19634] -
Unboiled various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ORP8 antibody (NBP2-19634) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
ORP8 Antibody [NBP2-19634] -
Analysis of methanol-fixed HeLa, using ORP8 antibody at 1:500 dilution.
Western Blot: ORP8 Antibody [NBP2-19634] -
Boiled and unboiled HeLa whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ORP8 antibody (NBP2-19634) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)Applications for ORP8 Antibody - Azide Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Format
Azide Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ORP8
Alternate Names
MGC126578, MGC133203, MST120, MSTP120, ORP-8, ORP8KIAA1451, OSBP10DKFZp686A11164, OSBP-related protein 8, oxysterol binding protein-like 8, oxysterol-binding protein-related protein 8
Gene Symbol
OSBPL8
UniProt
Additional ORP8 Products
Product Documents for ORP8 Antibody - Azide Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ORP8 Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for ORP8 Antibody - Azide Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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