p53 Transcription Factor Assay Kit (Colorimetric)

Name: p53 Transcription Factor Assay Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA1351
Price: 879 USD
Availability: InStock

Novus Biologicals | Catalog # KA1351

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Sample Type & Volume Required Per Well

Nuclear Extracts (10 uL/well. All samples should be assayed in duplicate at minimum.)

View all p53 Activity Assays »

Product Specifications

Assay Type

Colorimetric

Kit Type

Transcription Factor Assay Kit (Colorimetric)

Description

p53 Transcription Factor Assay Kit is a method for detecting specific transcription factor DNA binding activity in nuclear extracts.

Scientific Data Images for p53 Transcription Factor Assay Kit (Colorimetric)

p53 Assay Kit (Colorimetric) [KA1351] - Assay of p53 from nickel chloride stimulated HeLa cell nuclear extracts.

Kit Contents for p53 Transcription Factor Assay Kit (Colorimetric)

96-Well Cover Sheet
Polysorbate 20
Transcription Factor Antibody Binding Buffer (10x)
Transcription Factor Binding Assay Buffer (4x)
Transcription Factor Developing Solution
Transcription Factor Goat Anti-Mouse HRP Conjugate
Transcription Factor p53 96-Well Strip Plate
Transcription Factor p53 Competitor dsDNA
Transcription Factor p53 Positive Control
Transcription Factor p53 Primary Antibody
Transcription Factor Reagent A
Transcription Factor Stop Solution
Wash Buffer Concentrate (400x)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p53

The tumor suppressor protein, p53 is a transcription factor that is commonly referred to as the 'guardian of the genome' because of its crucial role in coordinating cellular responses to genotoxic stress.The tumor suppressor activity of p53 is mediated by a variety of mechanisms including cell cycle arrest, apoptosis, and cellular senescence. Approximately 50% of human cancers carry a mutation in the p53 gene; of those tumors that do not have a mutation in the p53 gene, a significant proportion of them have inactivated p53 by alternative mechanisms.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

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Certificate of Analysis

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Product Specific Notices for p53 Transcription Factor Assay Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.