Q: I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).

A: Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.

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Monkey Pappalysin-1/PAPP-A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-49863

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Product Documents

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all Pappalysin-1/PAPP-A ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Monkey

Description

Assay Length: 3 h 30 min

精度

Intra-Assay Precision (Precision within an assay) CV% 10

Inter-Assay Precision (Precision between assays) CV% 10

Recovery for Monkey Pappalysin-1/PAPP-A ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Scientific Data Images for Monkey Pappalysin-1/PAPP-A ELISA Kit (Colorimetric)

ELISA: Monkey Pappalysin-1/PAPP-A ELISA Kit (Colorimetric) [NBP3-49863]

Standard Curve Reference.

Kit Contents for Monkey Pappalysin-1/PAPP-A ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Certificate of Analysis
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Description
Reference Standard
Reference Standard & Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Pappalysin-1/PAPP-A

PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).

Long Name

Pregnancy-associated Plasma Protein 1

Alternate Names

ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1

Gene Symbol

PAPPA

Additional Pappalysin-1/PAPP-A Products

Product Documents for Monkey Pappalysin-1/PAPP-A ELISA Kit (Colorimetric)

Download Product Datasheets

Product Specific Notices for Monkey Pappalysin-1/PAPP-A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Metalloproteases and Regulators

Reproduction

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