PDAP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-20223

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDAP1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human PDAP1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for PDAP1 Antibody - BSA Free

PDAP1 Antibody

Western Blot-NBP3-20223-PDAP1 Antibody-

Western Blot-NBP3-20223-PDAP1 Antibody-Lane A: HeLa Whole Cell LysateLane B: U251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:26 kDa
PDAP1 Antibody

Immunoprecipitation: PDAP1 Antibody [NBP3-20223] -

Immunoprecipitation: PDAP1 Antibody [NBP3-20223] - PDAP1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 µL NBP3-20223 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-20223,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :26 kDa
PDAP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PDAP1 Antibody [NBP3-20223] -

Immunocytochemistry/ Immunofluorescence: PDAP1 Antibody [NBP3-20223] - PDAP1 Antibody-staining of PDAP1 in U251MG cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with NBP3-20223 (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm and cell membrane.

Applications for PDAP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5 uL/mg lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDAP1

Enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB (By similarity)

Alternate Names

HASPP28, PAP1PDGFA-associated protein 1, PAP28 kDa heat- and acid-stable phosphoprotein, PDGFA associated protein 1, PDGF-associated protein

Gene Symbol

PDAP1

Additional PDAP1 Products

Product Documents for PDAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PDAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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