Pea3 Antibody

Novus Biologicals | Catalog # NBP1-87960

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Pea3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pea3 Antibody

Immunocytochemistry/ Immunofluorescence: Pea3 Antibody [NBP1-87960]

Immunocytochemistry/ Immunofluorescence: Pea3 Antibody [NBP1-87960]

Immunocytochemistry/Immunofluorescence: Pea3 Antibody [NBP1-87960] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green. Antibody staining is shown in green.

Applications for Pea3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-87960 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pea3

Pea3, also known as ETS translocation variant 4, is an approximately 54 kDa, 484 amino acid protein, which is involved in transcriptional activation of adenovirus E1A gene, and is regulated by ERBB2, STK11, PLAG1, CTNNB1. Studies about the Pea3 protein are being done on diseases such as ovaria, pancreatic, gastric, breast, and prostate cancers. As well as peripheral primitive neuroectodermal tumors, carcinoma, adenoma, ewings sarcoma, squamous cell carcinoma, and oral cancers. The Pea3 protein has shown interaction with Taf7, EP300, SRF, HOXD4, STK11, and involvement in the ErbB2-ErbB3 Heterodimer pathway.

Alternate Names

Adenovirus E1A enhancer-binding protein, E1A-FE1A enhancer binding protein, E1AFETS translocation variant 4, ets variant 4, ets variant gene 4 (E1A enhancer binding protein, E1AF), ets variant gene 4 (E1A enhancer-binding protein, E1AF), EWS protein/E1A enhancer binding protein chimera, PEA3, PEAS3, Polyomavirus enhancer activator 3 homolog, polyomavirus enhancer activator-3, Protein PEA3

Gene Symbol

ETV4

Additional Pea3 Products

Product Documents for Pea3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Pea3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pea3 Antibody (1)

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1 Customer Rating
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Customer Images

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  • Pea3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: E11.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 01/07/2021
    Pea3 staining on E11.5 mouse Right Ventricle
    Fixed 4% PFA overnight. Blocked with 1% BSA Primary antibody dilution - 1:40 Secondary antibody - Invitrogen Alexa Fluor 488 Secondary antibody - 1:1000
    Pea3 Antibody NBP1-87960

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